| Rank | Team | Time | Year |
| 1 | Aubrey Runyon, Addie Martin, Hanna Stewart, Sami Frazier | 48.48 | 2025 |
| 2 | Aubrey Runyon, Addie Martin, Hanna Stewart, Sami Frazier | 48.76 | 2024 |
| 3 | Cami Kelsay, Rachel Simmons, Emma Beavins, Kloie Doublin | 49.38 | 2018 |
| 4 | Ellie Stringer, Jennifer Briggs, Brooke McClure, Erica Long | 49.82 | 1997 |
| 5 | Jennifer Stone, Christina Thompson, Kristen Chiarotti, Dawn Golden | 50.00 | 1989 |
| 6 | Morgan Hoy, Megan Woods, Kaitlyn Novak, Rachel Simmons | 50.84 | 2017 |
| 7 | Jennifer Stone, Chris Teike, Kristen Chairotti, Shannon Eades | 51.10 | 1988 |
| 8 | Natalie McWhorter, Randi Stock, Kylie Burgess, Erin Bullington | 51.30 | 2003 |
| 9 | Ellie Stringer, Brooke McClure, Jennifer Briggs, Andrea White | 51.60 | 1998 |
| 10 | Debbie Pruitt, Debbie Baker, Jamie Logan, Ann Golden | 51.69 | 1984 |
| 11 | Penny Humes, Lisa Olsen, Debbie Baker, Charlotte Linville | 51.70 | 1982 |
| 12 | Erica Long, Carrie Reese, Brooke McClure, Michelle Cummings | 51.70 | 1996 |
| 13 | Morgan Sandrock, Casey Macharia, Hailey Stewart, Samantha Frazier | 51.76 | 2023 |
| 14 | Aubrey Runyon, Ava Lenkey, Sabina Garcia, Olivia Nicoloff | 51.92 | 2022 |
| 15 | Katrina Emry, Kelly Clarkston, Jennifer Briggs, Natalie McWhorter | 52.04 | 2000 |
| 16 | Stacy Blotch, Brooke McClure, Brooke Clarkston, Erica Long | 52.10 | 1995 |
| 17 | Sarah Cearing, Angela Shaul, Kylie Burgess, Erin Bullington | 52.14 | 2004 |
| 18 | Jennifer Stone, Chris Teike, Kristen Chiarotti, Shannon Eades | 52.15 | 1987 |
| 19 | Jerica Richter, Angela Shaul, Erin Bullington, Kylie Burgess | 52.28 | 2006 |
| 20 | Sarah Cearing, Angela Shaul, Kylie Burgess, Erin Bullington | 52.31 | 2005 |
| 21 | Morgan Hoy, Megan Woods, Katie Novak, Rachel Simmons | 52.38 | 2016 |
| 22 | Shannon Eades, Jennifer Stone, Almeda Burton, Kristen Chiarotti | 52.40 | 1986 |
| 23 | Michelle Baker, Tammy Shatto, Jamie Logan, Shannon Eades | 52.48 | 1985 |
| 24 | Jerica Richter, Rebecca Allee, Destinee Smith, Tori Hamner | 52.53 | 2009 |
| 25 | Morgan Hoy, Carly Sandrock, Emma Beavins, Kloie Doublin | 52.58 | 2015 |
| Rank | Team | Time | Year |
| 1 | Carter Chitwood-Bedolla, Carlos Ogega, Jackson Frazier, Alex Leugers | 42.39 | 2025 |
| 2 | Aaron Scott, James Pfiefer, Jake Moran, Brian Murr | 42.86 | 2012 |
| 3 | Jackson Frazier, Clay Pinnick, Aidan Jefry, Alex Leugers | 43.23 | 2024 |
| 4 | Anthony Scott, James Pfifer, Andrew Ballay, Brian Murr | 43.26 | 2011 |
| 5 | Ryan Hall, Brian Shephard, Sean Packer, Richard Jordan | 43.50 | 1989 |
| 6 | Ethan Elsner, Elijah Smith, Aidan Jefry, Dylan Beverly | 43.77 | 2023 |
| 7 | Bart Hunter, Craig McCarty, Ja Martin, Jason Shephard | 43.90 | 1992 |
| Matt Giddens, Austin Clark, Clay Warner, Brian Murr | 43.90 | 2010 | |
| 9 | Richard Jordan, Sean Packer, Brian Shephard, Ryan Hall | 44.01 | 1988 |
| 10 | Nick Belser, Craig Thompson, Jason Wienhorst, Scott Whittenbarger | 44:04 | 1998 |
| 11 | Jeremy Price, Ben Yaden, Brad Epperson, Bart Hunter | 44.22 | 1993 |
| 12 | James Jones, Riley McCann, Javon Harris, Travis Murr | 44.23 | 2017 |
| 13 | Mike Sever, Eric Richards, Brad Mosburg, Bart Hunter | 44.68 | 1995 |
| 14 | Jake Kocher, Craig Thompson, Jason Wienhorst, Scott Whittenbarger | 44.75 | 1997 |
| 15 | Johntel Smith, Brian Murr, Chris Makransky, Jake Moran | 44.80 | 2009 |
| 16 | Myles Nickleson, Ashton Freese-Gastil, Aidan Jefry, Greg Hueston | 44.85 | 2022 |
| 17 | Kyler Gardner, Ranon Madison, Lukas Nibarger, Josh Williams | 45.10 | 2006 |
| 18 | Brad Epperson, Eric Richards, Ben Yaden, Mike Cowden | 45.10 | 1994 |
| 19 | Josh Cox, Michael Chadbourne, Adam Shepherd, Zach Dowell | 45.2 | 2014 |
| 20 | Aaron Loos, Chandler Mills, Kenny Hamblin, Riley McCann | 45.27 | 2018 |
| 21 | Drew Utterback, Tommy Reed, Josh Cox, Travis Murr | 45.32 | 2015 |
| 22 | Brook Baumgardner, Nick Hamner, Rob Turner, Chris Wagner | 45.36 | 2002 |
| 23 | Josh Deckard, Scott Whittenbarger, Josh Stevenson, Dustin Huddleston | 45.33 | 1999 |
| 24 | Lukas Nibarger, Ravin Montalvo, AJ Noblitt, Ranon Madison | 45.40 | 2005 |
| 25 | Zach Dowell, Adrew Ballay, Adam Shepherd, Josh Cox | 45.42 | 2013 |