| Rank | Name | Time | Year |
| 1 | Morgan Goode | 46.65 | 2002 |
| 2 | Ally Parramore | 47.06 | 2016 |
| 3 | C. Teike | 48.20 | 1986 |
| 4 | D. Murphy | 49.20 | 1985 |
| 5 | Courtney Clarkston | 49.30 | 1997 |
| 6 | M. Cummings | 49.40 | 1995 |
| 7 | Emma Beavins | 49.74 | 2018 |
| 8 | Brooke Coffman | 50.03 | 2021 |
| 9 | Lauren Sandrock | 50.28 | 2019 |
| 10 | Andrea White | 50.36 | 1997 |
| 11 | Hali Parker | 50.60 | 2009 |
| 12 | S. Mariani | 50.91 | 1998 |
| 13 | L. Baker | 51.09 | 1987 |
| 14 | Sarah Cearing | 51.42 | 2005 |
| 15 | Brittany Ward | 51.44 | 2012 |
| 16 | T. Spicer | 51.90 | 1994 |
| 17 | Jerica Richter | 52.31 | 2006 |
| 18 | Morgan Sandrock | 52.53 | 2021 |
| 19 | M. Spicer | 52.80 | 1991 |
| 20 | K. Gramse | 52.88 | 2002 |
| 21 | Samantha Woodall | 52.99 | 2019 |
| 22 | Carly Sandrock | 53.09 | 2015 |
| 23 | M. Craig | 53.18 | 2001 |
| 24 | A. Gibson | 53.50 | 2017 |
| 25 | Katie Gordon | 53.61 | 2004 |