| Rank | Name | Time | Year |
| 1 | Johntel Smith | 39.15 | 2009 |
| Sam Barnett | 39.15 | 2016 |
| 3 | Nick Riggles | 39.74 | 2018 |
| 4 | Josh Stevenson | 40.10 | 1999 |
| 5 | Rob Turner | 40.30 | 2003 |
| 6 | Andrew Ballay | 40.76 | 2013 |
| 7 | Aaron Williams | 41.10 | 2008 |
| 8 | Ranon Madison | 41.18 | 2006 |
| 9 | K. Walls | 41.50 | 2003 |
| 10 | Greg Hueston | 41.56 | 2021 |
| 11 | Nick Chupp | 42.14 | 2009 |
| 12 | Alex Gaddy | 42.43 | 2016 |
| 13 | Austin Armacost | 42.60 | 2003 |
| 14 | GT Jenkins | 42.62 | 2004 |
| 15 | D. Madison | 42.67 | 2010 |
| 16 | Gavin Wilson | 42.85 | 2021 |
| 17 | JD Sever | 43.09 | 2021 |
| 18 | Matt Wheatley | 43.50 | 1999 |
| 19 | Jason Maddox | 44.12 | 2007 |
| 20 | Matt Lamb | 44.50 | 2002 |
| 21 | I. Lauer | 44.51 | 2007 |
| 22 | Dominic Twardowski | 44.83 | 2014 |
| 23 | G. Koenig | 45.00 | 2011 |
| 24 | David Doan | 45.7 | 2019 |
| 25 | Quentin Gregory | 46.48 | 2019 |