| Rank | Team | Time | Year |
| 1 | Aubrey Runyon, Hanna Stewart, Ainsley Botkin, Lauren Klem | 3:55.47 | 2024 |
| 2 | Aubrey Runyon, Ainsley Botkin, Brooke Coffman, Lauren Klem | 4:03.85 | 2023 |
| 3 | Olivia Nicoloff, Ainsley Botkin, Brooke Coffman, Lauren Klem | 4:04.04 | 2022 |
| 4 | Sami Frazier, Hanna Stewart, Ainsley Botkin, Aubrey Runyon | 4:07.85 | 2025 |
| 5 | Ally Parramore, Emma Beavins, Kloie Doublin, McKinley Purdy | 4:07.99 | 2016 |
| 6 | Kloie Doublin, Ally Parramore, Maggie Auger, Emma Beavins | 4:09.72 | 2017 |
| 7 | Carrie Reese, Amy Stenger, Kelly Clarkston, Erica Long | 4:09.82 | 1997 |
| 8 | Alyssa Henderson, Lillian Lacy, Macy Doublin, Hailey Stewart | 4:13.19 | 2021 |
| 9 | Jennifer Burton, Christy Walters, Tina Spicer, Sarah Craciunoiu | 4:13.40 | 1994 |
| 10 | Christy Walters, Sarah Craciunoiu, Tina Spicer, Jennifer Burton | 4:13.60 | 1993 |
| 11 | Carrie Reese, Kimere Corthell, Suzanne Duckworth, Sara Moyer | 4:14.59 | 1998 |
| 12 | Erin Bullington, Katie Walls, Liz Clark, Kylie Burgess | 4:14.52 | 2005 |
| 13 | Carrie Reese, Michelle Cummings, Amy Stenger, Erica Long | 4:14.80 | 1996 |
| 14 | Morgan Goode, Jill Heminger, Ashley Gilles, Christi Shook | 4:15.47 | 2000 |
| 15 | Morgan Goode, Jill Heminger, Jenny Jessen, Christi Shook | 4:15.60 | 2001 |
| 16 | Rachel Cain, Morgan Goode, Ashley Gilles, Chrsti Shook | 4:15.90 | 1999 |
| 17 | Erin Bullington, Kylie Burgess, Ashley Bechman, Liz Clark | 4:16.11 | 2006 |
| 18 | Ally Parramore, Carly Sandrock, Khloie Doublin, McKinley Purdy | 4:16.61 | 2015 |
| 19 | Christi Shook, Ashley Adams, Rachel Cain, Morgan Goode | 4:17.86 | 2002 |
| 20 | McKinley Purdy, Alexis Hash, Mia Cano, Brittany Ward | 4:18.41 | 2013 |
| 21 | Maggie Auger, Liv Parramore, Chloe Selking, Emma Beavins | 4:18.56 | 2018 |
| 22 | Alyssa Henderson, Lauren Sandrock, Hailey Doddridge, Maggie Auger | 4:18.73 | 2019 |
| 23 | Lavonda Hendrickson, Debbie Baker, Debbie Pruitt, Cheryl Price | 4:20.50 | 1985 |
| 24 | Courtney Clarkston, Michelle Cummings, Amy Stenger, Carrie Reese | 4:20.70 | 1995 |
| 25 | Cheryl Price, Michelle Baker, Debbie Pruitt, Julie Carroll | 4:21.91 | 1986 |
| Rank | Team | Time | Year |
| 1 | Mark Findley, Bob Walters, Gordon Deskins, Ron Dougherty | 3:23.90# | 1969 |
| 2 | Riley Flora, Ethan Elsner, Joseph Judson, JD Sever | 3:25.82 | 2024 |
| 3 | Mike Hart, Bill Bensheimer, Rusty Huey, Tom Walters | 3:26.80# | 1965 |
| 4 | Nick Belser, Chris Duckworth, Jermey Coy, Josh Stevenson | 3:26.89 | 1998 |
| 5 | Noah Stevenson, Ethan Pheifer, Gavin Wilson, Greg Hueston | 3:27.87 | 2021 |
| 6 | Nick Belser, Kris Alexander, Jermey Coy, Keith Fechtman | 3:27.90 | 1997 |
| 7 | Sam Barnett, Jake Brown, Chandler McGaha, Tyler Fromer | 3:28.10 | 2016 |
| 8 | Joseph Judson, Gavin Fugate, Cooper Wienhorst, Corbin Shafer | 3:28.74 | 2025 |
| 9 | Austin Clark, James Pfifer, Clay Warner, Johntel Smith | 3:30.07 | 2009 |
| 10 | Austin Clark, Clay Warner, Ryan Atkison, James Pfifer | 3:30.19 | 2010 |
| 11 | Eric Crossman, Fred Brockman, Justin Webb, Larry Humbles | 3:30.30 | 1992 |
| 12 | David Purdy, Aaron Loos, Nick Riggles, Tyler Fromer | 3:30.63 | 2018 |
| 13 | Sam Barnett, Chandler McGaha, Jake Brown, Tyler Fromer | 3:30.88 | 2017 |
| 14 | Austin Clark, Cody McCarty, Andy Dunn, Ricky Warren | 3:31.13 | 2007 |
| 15 | Andy Dunn, Ranon Madison, Cody McCarty, Ricky Warren | 3:31.26 | 2006 |
| 16 | Mike Roach, Ike Adams, Troy Jarvis, Ed Reece | 3:31.30 | 1989 |
| 17 | Joel Compton, Andy Dunn, Ranon Madison, Rob Turner | 3:31.38 | 2005 |
| 18 | Kris Alexander, Jermey Coy, Chris Duckworth, Keith Fechtman | 3:31.67 | 1996 |
| 19 | Bruce Pierce, Derek DeBoy, Jason Wienhorst, Josh Stevenson | 3:32.64 | 1999 |
| 20 | Aidan Jefry, JD Sever, Ty Murphy, Greg Hueston | 3:32.86 | 2022 |
| 21 | Ari Smith, JD Sever, Jesse Toves, Ty Murphy | 3:33.06 | 2023 |
| 22 | Larry Humbles, Ryan Hall, Ed Reece, Brad Roberts | 3:33.10 | 1990 |
| 23 | Brian Gallagher, Rob Turner, Terrance Weaver, Kyle Walls | 3:33.70 | 2003 |
| 24 | Andy O'Neal, Brad Mosburg, Keith Fechtman, Scott White | 3:33.91 | 1995 |
| 25 | Eric Crossman, Shane Service, Fred Brockman, Larry Humbles | 3:34.49 | 1991 |
#Mile Relay Conversion |