| Rank | Name | Time | Year |
| 1 | Erica Long | 12.10 | 1997 |
| 2 | Dolly VanCleave | 12.42 | 1983 |
| 3 | Kloie Doublin | 12.46 | 2018 |
| 4 | Natalie McWhorter | 12.55 | 2002 |
| 5 | Addie Martin | 12.6 | 2024 |
| 6 | Olivia Nicoloff | 12.63 | 2022 |
| 7 | Anne Golden | 12.68 | 1984 |
| Cami Kelsay | 12.68 | 2018 |
| 9 | Jennifer Stone | 12.70 | 1988 |
| Ellie Stringer | 12.70 | 1997 |
| 11 | Erin Bullington | 12.74 | 2004 |
| Aubrey Runyon | 12.74 | 2023 |
| 13 | Samantha Frazier | 12.76 | 2023 |
| 14 | Christina Thompson | 12.80 | 1991 |
| 15 | Sue Repp | 12.90 | 1981 |
| 16 | Shannon Eades | 12.95 | 1988 |
| 17 | Rachel Simmons | 13.00 | 2018 |
| 18 | Lauren Klem | 13.06 | 2024 |
| 19 | Jennifer Briggs | 13.09 | 2000 |
| 20 | Andrea White | 13.10 | 1998 |
| 21 | Brooke McClure | 13.20 | 1997 |
| Angela Shaul | 13.20 | 2005 |
| Tori Hamner | 13.20 | 2011 |
| Liz Clark | 13.20 | 2009 |
| Rank | Name | Time | Year |
| 1 | Richard Jordan | 10.50 | 1988 |
| 2 | Bart Hunter | 10.80 | 1993 |
| Keith Fechtman | 10.80 | 1997 |
| Brian Murr | 10.80 | 2011 |
| 5 | Jerry Broaddus | 10.9* | 1961 |
| 6 | Alex Leugers | 10.93 | 2024 |
| 7 | Gary White | 11.00 | 1981 |
| 8 | Travis Murr | 11.02 | 2017 |
| 9 | Lukas Nibarger | 11.10 | 2006 |
| Brent Waymire | 11.10* | 1972 |
| Mark Findley | 11.10* | 1969 |
| Ron Dougherty | 11.10* | 1967 |
| 13 | Nick Hamner | 11.20 | 2003 |
| Andy Dunn | 11.20 | 2006 |
| Jake Moran | 11.20 | 2009 |
| Josh Cox | 11.20 | 2014 |
| 17 | Ja Martin | 11.25 | 1992 |
| Dylan Beverly | 11.25 | 2023 |
| 19 | Riley McCann | 11.28 | 2018 |
| 20 | Scott Whittenbarger | 11.29 | 1998 |
| 21 | Brian Shepard | 11.30 | 1988 |
| 22 | Jeremy Price | 11.32 | 1993 |
| Jackson Frazier | 11.32 | 2024 |
| 24 | Sean Hamner | 11.33 | 2008 |
| 25 | Craig Thompson | 11.35 | 1998 |
| *=Converted times | |